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Assay Detail

CHEMBL5517420

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiviral activity against SARS CoV-2 spike-pseudotyped lentivirus infected in human A549-hACE2-TMPRSS2 cells assessed as inhibition of viral entry incubated for 24 hrs in absence of BSA by luciferase reporter assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Severe acute respiratory syndrome coronavirus 2
Cell Type A549-hACE2-TMPRSS2
Confidence 1 — Organism
Curated By Autocuration

Target

SARS-CoV-2 (CHEMBL4303835)
Type ORGANISM
Organism Severe acute respiratory syndrome coronavirus 2

Publication

Colloidal Aggregation Confounds Cell-Based Covid-19 Antiviral Screens.
Glenn IS, Hall LN, Khalid MM, Ott M, Shoichet BK.
J Med Chem (2024) 67 : 10263 -10274
PubMed 38864383 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 9 5.56 7.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL449947 1 7.16
CHEMBL1214598 UMIFENOVIR 3.0 1 6.58
CHEMBL1336 SORAFENIB 4.0 1 6.39
CHEMBL463843 CANNABIGEROLIC ACID 1 5.92
CHEMBL289277 EMODIN 1 5.66
CHEMBL262344 BAKUCHIOL 1.0 1 5.27
CHEMBL639 AZELASTINE 4.0 1 4.57
CHEMBL297453 EPIGALOCATECHIN GALLATE 3.0 1 4.46
CHEMBL286494 HYPERICIN 3.0 1 4.07

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL449947 EC50 = 70.0 nM 7.16
CHEMBL1214598 UMIFENOVIR EC50 = 260.0 nM 6.58
CHEMBL1336 SORAFENIB EC50 = 410.0 nM 6.39
CHEMBL463843 CANNABIGEROLIC ACID EC50 = 1210.0 nM 5.92
CHEMBL289277 EMODIN EC50 = 2210.0 nM 5.66
CHEMBL262344 BAKUCHIOL EC50 = 5350.0 nM 5.27
CHEMBL639 AZELASTINE EC50 = 27000.0 nM 4.57
CHEMBL297453 EPIGALOCATECHIN GALLATE EC50 = 35100.0 nM 4.46
CHEMBL286494 HYPERICIN EC50 = 84400.0 nM 4.07