Assay Detail
Binding
CHEMBL5519218
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Inhibition of N-terminal 6His-TEV-tagged recombinant human GPX4-U46C-C10A-C66A mutant (Met1 to Phe170 residues) expressed in Escherichia coli BL21 (DE3) cells incubated for 20 mins by fluorescence polarization based high-throughput screening assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Discovery of GPX4 inhibitors through FP-based high-throughput screening.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 5.27 | 5.85 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL487894 | DIPYRONE SODIUM | — | 1 | 5.85 |
| CHEMBL5083184 | — | — | 1 | 5.34 |
| CHEMBL442687 | TIOXOLONE | 2.0 | 1 | 5.29 |
| CHEMBL4218391 | — | — | 1 | 5.18 |
| CHEMBL1486563 | — | — | 1 | 5.12 |
| CHEMBL1473252 | SODIUM TANSHINONE IIA SULFONATE | 2.0 | 1 | 4.86 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL487894 | DIPYRONE SODIUM | IC50 | = | 1400.0 | nM | 5.85 |
| CHEMBL5083184 | — | IC50 | = | 4580.0 | nM | 5.34 |
| CHEMBL442687 | TIOXOLONE | IC50 | = | 5180.0 | nM | 5.29 |
| CHEMBL4218391 | — | IC50 | = | 6540.0 | nM | 5.18 |
| CHEMBL1486563 | — | IC50 | = | 7520.0 | nM | 5.12 |
| CHEMBL1473252 | SODIUM TANSHINONE IIA SULFONATE | IC50 | = | 13850.0 | nM | 4.86 |