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Assay Detail

CHEMBL5523024

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Anti-inflammatory activity in mouse macrophages extracted from abdominal activity assessed as inhibition of LPS-induced nitric oxide production at 1 uM measured after 22 hrs by Griess reagent assay relative to control
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type Macrophage
Confidence 1 — Organism
Curated By Autocuration

Target

Macrophage (CHEMBL5314319)
Type CELL-LINE
Organism Mus musculus

Publication

Design, Synthesis, and Biological Evaluation of a Novel NIK Inhibitor with Anti-Inflammatory and Hepatoprotective Effects for Sepsis Treatment.
Zhang N, Shen S, Yang M, He S, Liu C, Li H, Lu T, Liu H, Hu Q, Tang W, Chen Y.
J Med Chem (2024) 67 : 5617 -5641
PubMed 38563549 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 4.32 4.78

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5567931 1 4.78
CHEMBL5542810 1 4.76
CHEMBL5542106 1 4.34
CHEMBL5564890 1 4.23
CHEMBL3187788 1 4.23
CHEMBL5523606 1 4.19
CHEMBL5542501 1 4.12
CHEMBL5562073 1 4.11
CHEMBL5556543 1 4.09

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5567931 IC50 = 16500.0 nM 4.78
CHEMBL5542810 IC50 = 17200.0 nM 4.76
CHEMBL5542106 IC50 = 45900.0 nM 4.34
CHEMBL5564890 IC50 = 59400.0 nM 4.23
CHEMBL3187788 IC50 = 58600.0 nM 4.23
CHEMBL5523606 IC50 = 64100.0 nM 4.19
CHEMBL5542501 IC50 = 76800.0 nM 4.12
CHEMBL5562073 IC50 = 78100.0 nM 4.11
CHEMBL5556543 IC50 = 80300.0 nM 4.09