Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5524212

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human NHE5 transfected in chinese hamster PS120 cells measured after 9 mins by BCECF-AM staining based NH4Cl pre-pulse method
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type PS120
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium/hydrogen exchanger 5 (CHEMBL3058)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of UTX-143, a selective sodium-hydrogen exchange subtype 5 inhibitor, using amiloride as a lead compound.
Shinohara Y, Komiya Y, Morimoto K, Endo Y, Terashima M, Suzuki T, Takino T, Ninomiya I, Yamada H, Uto Y.
Bioorg Med Chem (2024) 99 : 117603 -117603
PubMed 38246115 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 4.72 5.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL501701 N,N-HEXAMETHYLENEAMILORIDE 1 5.82
CHEMBL5565656 1 5.51
CHEMBL5568323 1 5.34
CHEMBL5542750 1 5.32
CHEMBL5560204 1 5.25
CHEMBL5567224 1 5.05
CHEMBL5556438 1 4.96
CHEMBL5563696 1 4.80
CHEMBL5563134 1 4.79
CHEMBL5561147 1 4.74
CHEMBL5555889 1 4.66
CHEMBL5560463 1 4.52
CHEMBL5559174 1 4.52
CHEMBL5562697 1 4.36
CHEMBL945 AMILORIDE 4.0 1 4.30
CHEMBL5561432 1 4.26
CHEMBL5555508 1 4.16
CHEMBL5560348 1 4.00
CHEMBL5566603 1 4.00
CHEMBL5559101 1 4.00
CHEMBL5558607 1 -
CHEMBL5566999 1 -
CHEMBL5562282 1 -
CHEMBL5556133 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL501701 N,N-HEXAMETHYLENEAMILORIDE IC50 = 1510.0 nM 5.82
CHEMBL5565656 IC50 = 3110.0 nM 5.51
CHEMBL5568323 IC50 = 4540.0 nM 5.34
CHEMBL5542750 IC50 = 4750.0 nM 5.32
CHEMBL5560204 IC50 = 5580.0 nM 5.25
CHEMBL5567224 IC50 = 8830.0 nM 5.05
CHEMBL5556438 IC50 = 11100.0 nM 4.96
CHEMBL5563696 IC50 = 15900.0 nM 4.80
CHEMBL5563134 IC50 = 16200.0 nM 4.79
CHEMBL5561147 IC50 = 18300.0 nM 4.74
CHEMBL5555889 IC50 = 22000.0 nM 4.66
CHEMBL5560463 IC50 = 30100.0 nM 4.52
CHEMBL5559174 IC50 = 30100.0 nM 4.52
CHEMBL5562697 IC50 = 43400.0 nM 4.36
CHEMBL945 AMILORIDE IC50 = 49500.0 nM 4.30
CHEMBL5561432 IC50 = 55100.0 nM 4.26
CHEMBL5555508 IC50 = 69500.0 nM 4.16
CHEMBL5560348 IC50 = 100000.0 nM 4.00
CHEMBL5566603 IC50 = 100000.0 nM 4.00
CHEMBL5559101 IC50 = 100000.0 nM 4.00
CHEMBL5558607 IC50 > 100000.0 nM -
CHEMBL5566999 IC50 > 100000.0 nM -
CHEMBL5562282 IC50 > 100000.0 nM -
CHEMBL5556133 IC50 > 10000.0 nM -