Assay Detail
Binding
CHEMBL5524265
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human N-terminal His6-tagged FIH (M1 to N349 residues) expressed in Escherichia coli BL21 (DE3) using HIF-1alpha-CAD peptide as substrate preincubated for 15 mins followed by substrate and 2-OG addition measured after 15 mins by SPE-MS analysis
3
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Selective targeting of human TET1 by cyclic peptide inhibitors: Insights from biochemical profiling.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| pIC50 | 2 | - | - |
| IC50 | 1 | 4.83 | 4.83 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5557372 | — | — | 1 | 4.83 |
| CHEMBL1230640 | — | — | 1 | - |
| CHEMBL5542589 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5557372 | — | IC50 | = | 14791.08 | nM | 4.83 |
| CHEMBL1230640 | — | pIC50 | - | — | - | |
| CHEMBL5542589 | — | pIC50 | - | — | - |