Assay Detail
Binding
CHEMBL5525872
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Positive allosteric modulation of GCase in human Fibroblast cells using GBA1-FQ2 as substrate preincubated for 24 hrs followed by substrate addition and measured after 3 hrs by Hoechst 33342 staining based imaging analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Fibroblast |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Fragment-Based Discovery of a Series of Allosteric-Binding Site Modulators of β-Glucocerebrosidase.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 4 | 4.77 | 5.05 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5560927 | — | — | 1 | 5.05 |
| CHEMBL5562071 | — | — | 1 | 5.05 |
| CHEMBL153479 | AMBROXOL | 3.0 | 1 | 4.53 |
| CHEMBL5567650 | — | — | 1 | 4.43 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5560927 | — | EC50 | = | 8900.0 | nM | 5.05 |
| CHEMBL5562071 | — | EC50 | = | 9000.0 | nM | 5.05 |
| CHEMBL153479 | AMBROXOL | EC50 | = | 29400.0 | nM | 4.53 |
| CHEMBL5567650 | — | EC50 | = | 37100.0 | nM | 4.43 |