Assay Detail
Binding
CHEMBL5531847
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of His-tagged human recombinant wild type RET M918T mutant expressed in Sf9 cells using poly(Glu, Tyr) peptide as substrate in presence of ATP preincubated fo4 30 mins followed by substrate addition and measured after 1 hr by FRET analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Proto-oncogene tyrosine-protein kinase receptor Ret (CHEMBL2041) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Unraveling the Promise of RET Inhibitors in Precision Cancer Therapy by Targeting RET Mutations.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 9.40 | 9.40 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4582651 | PRALSETINIB | 4.0 | 1 | 9.40 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4582651 | PRALSETINIB | IC50 | = | 0.4 | nM | 9.40 |