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Assay Detail

CHEMBL5534151

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human His-tagged CDK7/cyclin H/MNAT1 expressed in baculovirus infected insect cells preincubated for 10 mins followed by substrate and ATP addition and measured after 60 mins by ADP-Glo kinase assay
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Insect cell
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

CDK7/Cyclin H/MNAT1 (CHEMBL3038473)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery and optimization of thieno[3,2-d]pyrimidine derivatives as highly selective inhibitors of cyclin-dependent kinase 7.
Zhang H, Lin G, Jia S, Zhang Y, Wu J, Tao Y, Huang W, Song M, Ding K, Ma D, Fan M.
Eur J Med Chem (2024) 263 : 115955 -115955
PubMed 38000213 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 7.56 8.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5090754 SY-5609 1.0 1 8.60
CHEMBL5574236 1 8.21
CHEMBL5574001 1 8.19
CHEMBL5569504 1 8.14
CHEMBL5573695 1 8.14
CHEMBL5573538 1 8.06
CHEMBL5594350 1 8.06
CHEMBL5570731 1 8.05
CHEMBL5542311 1 8.00
CHEMBL5572618 1 7.99
CHEMBL5594861 1 7.96
CHEMBL5574040 1 7.65
CHEMBL5564846 1 7.60
CHEMBL5569637 1 7.58
CHEMBL5592607 1 7.46
CHEMBL5571051 1 7.42
CHEMBL5572978 1 7.40
CHEMBL5590200 1 7.28
CHEMBL5567015 1 7.24
CHEMBL5570965 1 7.24
CHEMBL5569741 1 7.19
CHEMBL5568630 1 7.14
CHEMBL5592891 1 7.11
CHEMBL5575677 1 7.06
CHEMBL5595915 1 7.05
CHEMBL5573083 1 7.02
CHEMBL5562849 1 6.86
CHEMBL5572474 1 6.85
CHEMBL5572331 1 6.66
CHEMBL5562749 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5090754 SY-5609 IC50 = 2.5 nM 8.60
CHEMBL5574236 IC50 = 6.1 nM 8.21
CHEMBL5574001 IC50 = 6.5 nM 8.19
CHEMBL5569504 IC50 = 7.2 nM 8.14
CHEMBL5573695 IC50 = 7.3 nM 8.14
CHEMBL5573538 IC50 = 8.8 nM 8.06
CHEMBL5594350 IC50 = 8.7 nM 8.06
CHEMBL5570731 IC50 = 8.9 nM 8.05
CHEMBL5542311 IC50 = 10.1 nM 8.00
CHEMBL5572618 IC50 = 10.2 nM 7.99
CHEMBL5594861 IC50 = 11.0 nM 7.96
CHEMBL5574040 IC50 = 22.6 nM 7.65
CHEMBL5564846 IC50 = 25.2 nM 7.60
CHEMBL5569637 IC50 = 26.0 nM 7.58
CHEMBL5592607 IC50 = 34.9 nM 7.46
CHEMBL5571051 IC50 = 37.8 nM 7.42
CHEMBL5572978 IC50 = 39.7 nM 7.40
CHEMBL5590200 IC50 = 52.4 nM 7.28
CHEMBL5567015 IC50 = 57.9 nM 7.24
CHEMBL5570965 IC50 = 57.0 nM 7.24
CHEMBL5569741 IC50 = 65.1 nM 7.19
CHEMBL5568630 IC50 = 72.4 nM 7.14
CHEMBL5592891 IC50 = 78.3 nM 7.11
CHEMBL5575677 IC50 = 86.7 nM 7.06
CHEMBL5595915 IC50 = 89.5 nM 7.05
CHEMBL5573083 IC50 = 95.0 nM 7.02
CHEMBL5562849 IC50 = 139.0 nM 6.86
CHEMBL5572474 IC50 = 142.0 nM 6.85
CHEMBL5572331 IC50 = 218.0 nM 6.66
CHEMBL5562749 IC50 > 2000.0 nM -