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Assay Detail

CHEMBL5534958

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human alpha3beta4 nAChR expressed in HEK293 cells assessed as inhibition of nicotine induced calcium flux preincubated for 10 mins followed by nicotine addition and measured for 5 mins by FLIPR assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha3/beta4 (CHEMBL1907594)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Synthesis of α3β4 Nicotinic Acetylcholine Receptor Modulators Derived from Aristoquinoline That Reduce Reinstatement of Cocaine-Seeking Behavior.
Rusali LE, Lopez-Hernandez AM, Kremiller KM, Kulkarni GC, Gour A, Straub CJ, Argade MD, Peters CJ, Sharma A, Toll L, Cippitelli A, Riley AP.
J Med Chem (2024) 67 : 529 -542
PubMed 38151460 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.88 6.25

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5572105 1 6.25
CHEMBL5571457 1 5.98
CHEMBL5595517 1 5.96
CHEMBL5574911 1 5.92
CHEMBL5569258 1 5.84
CHEMBL5572459 1 5.83
CHEMBL5570620 1 5.80
CHEMBL5575320 1 5.67
CHEMBL5572736 1 5.67

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5572105 IC50 = 560.0 nM 6.25
CHEMBL5571457 IC50 = 1050.0 nM 5.98
CHEMBL5595517 IC50 = 1100.0 nM 5.96
CHEMBL5574911 IC50 = 1210.0 nM 5.92
CHEMBL5569258 IC50 = 1460.0 nM 5.84
CHEMBL5572459 IC50 = 1470.0 nM 5.83
CHEMBL5570620 IC50 = 1570.0 nM 5.80
CHEMBL5575320 IC50 = 2120.0 nM 5.67
CHEMBL5572736 IC50 = 2130.0 nM 5.67