Assay Detail
Binding
CHEMBL5538801
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human DHFR using DHF as substrate measured for 1 hr in presence of NADPH by absorbance based assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Investigating the anti-cancer potential of pyrimethamine analogues through a modern chemical biology lens.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 11 | 4.89 | 5.26 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL586281 | — | — | 1 | 5.26 |
| CHEMBL36 | PYRIMETHAMINE | 4.0 | 1 | 5.17 |
| CHEMBL21414 | — | — | 1 | 5.09 |
| CHEMBL5575210 | — | — | 1 | 4.88 |
| CHEMBL5569615 | — | — | 1 | 4.76 |
| CHEMBL5569327 | — | — | 1 | 4.65 |
| CHEMBL5565442 | — | — | 1 | 4.40 |
| CHEMBL5575305 | — | — | 1 | - |
| CHEMBL5569919 | — | — | 1 | - |
| CHEMBL5575876 | — | — | 1 | - |
| CHEMBL5574847 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL586281 | — | IC50 | = | 5500.0 | nM | 5.26 |
| CHEMBL36 | PYRIMETHAMINE | IC50 | = | 6700.0 | nM | 5.17 |
| CHEMBL21414 | — | IC50 | = | 8170.0 | nM | 5.09 |
| CHEMBL5575210 | — | IC50 | = | 13200.0 | nM | 4.88 |
| CHEMBL5569615 | — | IC50 | = | 17500.0 | nM | 4.76 |
| CHEMBL5569327 | — | IC50 | = | 22600.0 | nM | 4.65 |
| CHEMBL5565442 | — | IC50 | = | 40000.0 | nM | 4.40 |
| CHEMBL5575305 | — | IC50 | > | 100000.0 | nM | - |
| CHEMBL5569919 | — | IC50 | - | — | - | |
| CHEMBL5575876 | — | IC50 | - | — | - | |
| CHEMBL5574847 | — | IC50 | > | 100000.0 | nM | - |