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Assay Detail

CHEMBL5541341

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of wild type FGFR3 (unknown origin) using 5-FAM-KKKKEEIYFFF-CONH2 peptide as fluorogenic substrate incubated for 80 mins in presence of ATP by fluorescence-based microfluidic mobility shift assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fibroblast growth factor receptor 3 (CHEMBL2742)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of KIN-3248, An Irreversible, Next Generation FGFR Inhibitor for the Treatment of Advanced Tumors Harboring FGFR2 and/or FGFR3 Gene Alterations.
Tyhonas JS, Arnold LD, Cox JM, Franovic A, Gardiner E, Grandinetti K, Kania R, Kanouni T, Lardy M, Li C, Martin ES, Miller N, Mohan A, Murphy EA, Perez M, Soroceanu L, Timple N, Uryu S, Womble S, Kaldor SW.
J Med Chem (2024) 67 : 1734 -1746
PubMed 38267212 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 8.01 9.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5593760 1 9.05
CHEMBL5572910 1 8.44
CHEMBL5572491 1 8.23
CHEMBL5592900 1 8.23
CHEMBL5571671 1 8.12
CHEMBL5568827 1 8.05
CHEMBL5589734 1 8.03
CHEMBL5314537 RESIGRATINIB 2.0 1 8.03
CHEMBL5594856 1 8.01
CHEMBL5574600 1 7.98
CHEMBL5572986 1 7.90
CHEMBL5569299 1 7.81
CHEMBL5566049 1 7.79
CHEMBL5563793 1 7.78
CHEMBL5566156 1 7.74
CHEMBL5572840 1 7.67
CHEMBL5569392 1 7.26
CHEMBL5570727 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5593760 IC50 = 0.9 nM 9.05
CHEMBL5572910 IC50 = 3.6 nM 8.44
CHEMBL5572491 IC50 = 5.9 nM 8.23
CHEMBL5592900 IC50 = 5.9 nM 8.23
CHEMBL5571671 IC50 = 7.6 nM 8.12
CHEMBL5568827 IC50 = 8.9 nM 8.05
CHEMBL5589734 IC50 = 9.4 nM 8.03
CHEMBL5314537 RESIGRATINIB IC50 = 9.3 nM 8.03
CHEMBL5594856 IC50 = 9.8 nM 8.01
CHEMBL5574600 IC50 = 10.4 nM 7.98
CHEMBL5572986 IC50 = 12.5 nM 7.90
CHEMBL5569299 IC50 = 15.4 nM 7.81
CHEMBL5566049 IC50 = 16.2 nM 7.79
CHEMBL5563793 IC50 = 16.7 nM 7.78
CHEMBL5566156 IC50 = 18.4 nM 7.74
CHEMBL5572840 IC50 = 21.2 nM 7.67
CHEMBL5569392 IC50 = 54.6 nM 7.26
CHEMBL5570727 IC50 - -