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Assay Detail

CHEMBL5544044

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of porcine pancreatic Alpha-amylase using starch as substrate preincubated for 10 mins followed by substrate addition and measured after 15 mins by Bio-rad microplate reader analysis
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Sus scrofa
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Alpha-amylase (CHEMBL3308960)
Type SINGLE PROTEIN
Organism Sus scrofa domesticus

Publication

3,5-Disubstituted-thiazolidine-2,4-dione hybrids as antidiabetic agents: Design, synthesis, in-vitro and In vivo evaluation.
Singh G, Singh R, Monga V, Mehan S.
Eur J Med Chem (2024) 266 : 116139 -116139
PubMed 38252989 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 4.43 4.99

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5570132 1 4.99
CHEMBL404271 ACARBOSE 4.0 1 4.65
CHEMBL5593340 1 4.62
CHEMBL5570061 1 4.62
CHEMBL5590690 1 4.60
CHEMBL5570680 1 4.55
CHEMBL5568913 1 4.52
CHEMBL5595761 1 4.52
CHEMBL5595692 1 4.51
CHEMBL5568664 1 4.41
CHEMBL5574059 1 4.39
CHEMBL5571680 1 4.37
CHEMBL5590029 1 4.35
CHEMBL5575399 1 4.35
CHEMBL5573604 1 4.32
CHEMBL5565970 1 4.10
CHEMBL5583596 1 4.09
CHEMBL5573635 1 4.08
CHEMBL5570624 1 4.08
CHEMBL196 ASCORBIC ACID 4.0 1 -
CHEMBL5570268 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5570132 IC50 = 10190.0 nM 4.99
CHEMBL404271 ACARBOSE IC50 = 22570.0 nM 4.65
CHEMBL5593340 IC50 = 24070.0 nM 4.62
CHEMBL5570061 IC50 = 23910.0 nM 4.62
CHEMBL5590690 IC50 = 25150.0 nM 4.60
CHEMBL5570680 IC50 = 27960.0 nM 4.55
CHEMBL5568913 IC50 = 30430.0 nM 4.52
CHEMBL5595761 IC50 = 30220.0 nM 4.52
CHEMBL5595692 IC50 = 30820.0 nM 4.51
CHEMBL5568664 IC50 = 39320.0 nM 4.41
CHEMBL5574059 IC50 = 40550.0 nM 4.39
CHEMBL5571680 IC50 = 42860.0 nM 4.37
CHEMBL5590029 IC50 = 44620.0 nM 4.35
CHEMBL5575399 IC50 = 44730.0 nM 4.35
CHEMBL5573604 IC50 = 48120.0 nM 4.32
CHEMBL5565970 IC50 = 79130.0 nM 4.10
CHEMBL5583596 IC50 = 81090.0 nM 4.09
CHEMBL5573635 IC50 = 82240.0 nM 4.08
CHEMBL5570624 IC50 = 82240.0 nM 4.08
CHEMBL196 ASCORBIC ACID IC50 - -
CHEMBL5570268 IC50 = 117830.0 nM -