Assay Detail
Binding
CHEMBL5546287
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of Histone H3 peptide from N-terminal Nanoluciferase fusion of full length SPIN1 (unknown origin) transfected in U2OS cells cotransfected with C-terminal Halo tagged-Histone 3.3 preincubated for 24 hrs followed by Nano-Glo substrate addition by NanoBRET assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | U2OS |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Discovery of a Potent, Selective, and Cell-Active SPIN1 Inhibitor.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 1 | 5.96 | 5.96 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5594627 | — | — | 1 | 5.96 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5594627 | — | EC50 | = | 1100.0 | nM | 5.96 |