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Assay Detail

CHEMBL5547050

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human full-length DNA-PK using EPPLSQEAFADLWKK as substrate incubated for 60 mins in presence of ATP by ADP-Glo kinase assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

DNA-dependent protein kinase catalytic subunit (CHEMBL3142)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Novel Heterotricyclic Compounds as DNA-Dependent Protein Kinase (DNA-PK) Inhibitors with Enhanced Chemosensitivity, Oral Bioavailability, and the Ability to Potentiate Cancer Immunotherapy.
Cheng B, Shi Y, Shao C, Wang S, Su Z, Liu J, Zhou Y, Fei X, Pan W, Chen J, Lu Y, Xiao J.
J Med Chem (2024) 67 : 6253 -6267
PubMed 38587857 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 8.23 9.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5570653 1 9.96
CHEMBL5575934 1 9.46
CHEMBL5573665 1 9.21
CHEMBL5191459 1 9.10
CHEMBL4439259 AZD-7648 1.0 1 8.92
CHEMBL5566235 1 8.44
CHEMBL5575262 1 8.25
CHEMBL5594841 1 8.08
CHEMBL5574154 1 8.05
CHEMBL5573849 1 7.82
CHEMBL5573920 1 7.67
CHEMBL5573858 1 7.58
CHEMBL5574170 1 7.53
CHEMBL5570510 1 7.29
CHEMBL5575434 1 7.20
CHEMBL5575062 1 7.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5570653 IC50 = 0.11 nM 9.96
CHEMBL5575934 IC50 = 0.35 nM 9.46
CHEMBL5573665 IC50 = 0.62 nM 9.21
CHEMBL5191459 IC50 = 0.8 nM 9.10
CHEMBL4439259 AZD-7648 IC50 = 1.2 nM 8.92
CHEMBL5566235 IC50 = 3.6 nM 8.44
CHEMBL5575262 IC50 = 5.6 nM 8.25
CHEMBL5594841 IC50 = 8.4 nM 8.08
CHEMBL5574154 IC50 = 8.9 nM 8.05
CHEMBL5573849 IC50 = 15.3 nM 7.82
CHEMBL5573920 IC50 = 21.4 nM 7.67
CHEMBL5573858 IC50 = 26.1 nM 7.58
CHEMBL5574170 IC50 = 29.4 nM 7.53
CHEMBL5570510 IC50 = 51.8 nM 7.29
CHEMBL5575434 IC50 = 62.8 nM 7.20
CHEMBL5575062 IC50 = 71.5 nM 7.15