Assay Detail
Binding
CHEMBL5548164
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Inhibition of wild type HIV-1 Reverse transcriptase assessed as reduction of biotin-dUTP incorporation into protein using ABTS as substrate preincubated with compound for 1 hr followed by substrate addition in presence of biotin-labeled dNTPs by ELISA analysis
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Human immunodeficiency virus 1 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Human immunodeficiency virus type 1 reverse transcriptase (CHEMBL247) ↗| Type | SINGLE PROTEIN |
| Organism | Human immunodeficiency virus 1 |
Publication
Discovery of Novel Aryl Triazolone Dihydropyridines (ATDPs) Targeting Highly Conserved Residue W229 as Promising HIV-1 NNRTIs.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 7.45 | 7.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5593560 | — | — | 1 | 7.70 |
| CHEMBL5575200 | — | — | 1 | 7.52 |
| CHEMBL5571067 | — | — | 1 | 7.52 |
| CHEMBL5573421 | — | — | 1 | 7.52 |
| CHEMBL5573653 | — | — | 1 | 7.40 |
| CHEMBL5564750 | — | — | 1 | 7.40 |
| CHEMBL5575003 | — | — | 1 | 7.40 |
| CHEMBL5563055 | — | — | 1 | 7.10 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5593560 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL5575200 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL5571067 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL5573421 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL5573653 | — | IC50 | = | 40.0 | nM | 7.40 |
| CHEMBL5564750 | — | IC50 | = | 40.0 | nM | 7.40 |
| CHEMBL5575003 | — | IC50 | = | 40.0 | nM | 7.40 |
| CHEMBL5563055 | — | IC50 | = | 80.0 | nM | 7.10 |