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Assay Detail

CHEMBL5550260

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal GST-tagged recombinant human MKK3 S198E/T193E mutant expressed in baculovirus infected Sf9 insect cells using STK-3-biotin as substrate preincubated for 30 mins followed by substrate/ATP addition measured after 90 mins by TR-FRET assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of pyrazolo[3,4-d]pyrimidines as novel mitogen-activated protein kinase kinase 3 (MKK3) inhibitors.
Takarada JE, Cunha MR, Almeida VM, Vasconcelos SNS, Santiago AS, Godoi PH, Salmazo A, Ramos PZ, Fala AM, de Souza LR, Da Silva IEP, Bengtson MH, Massirer KB, Couñago RM.
Bioorg Med Chem (2024) 98 : 117561 -117561
PubMed 38157838 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 24 5.15 5.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3545097 SAPANISERTIB 2.0 1 5.96
CHEMBL1241674 1 5.82
CHEMBL5570263 1 5.34
CHEMBL573578 1 5.32
CHEMBL5570047 1 5.21
CHEMBL5564326 1 5.19
CHEMBL264406 1 5.16
CHEMBL5571520 1 5.15
CHEMBL5573208 1 5.03
CHEMBL5567024 1 5.00
CHEMBL5568922 1 4.96
CHEMBL5566622 1 4.82
CHEMBL5574120 1 4.56
CHEMBL1232415 1 4.55
CHEMBL5595378 1 -
CHEMBL5569984 1 -
CHEMBL2079165 1 -
CHEMBL5590276 1 -
CHEMBL5565275 1 -
CHEMBL5571929 1 -
CHEMBL5570067 1 -
CHEMBL5563661 1 -
CHEMBL5565866 1 -
CHEMBL2078794 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3545097 SAPANISERTIB Ki = 1100.0 nM 5.96
CHEMBL1241674 Ki = 1500.0 nM 5.82
CHEMBL5570263 Ki = 4600.0 nM 5.34
CHEMBL573578 Ki = 4800.0 nM 5.32
CHEMBL5570047 Ki = 6200.0 nM 5.21
CHEMBL5564326 Ki = 6500.0 nM 5.19
CHEMBL264406 Ki = 7000.0 nM 5.16
CHEMBL5571520 Ki = 7100.0 nM 5.15
CHEMBL5573208 Ki = 9300.0 nM 5.03
CHEMBL5567024 Ki = 9900.0 nM 5.00
CHEMBL5568922 Ki = 11000.0 nM 4.96
CHEMBL5566622 Ki = 15000.0 nM 4.82
CHEMBL5574120 Ki = 27500.0 nM 4.56
CHEMBL1232415 Ki = 27900.0 nM 4.55
CHEMBL5595378 Ki - -
CHEMBL5569984 Ki - -
CHEMBL2079165 Ki - -
CHEMBL5590276 Ki - -
CHEMBL5565275 Ki - -
CHEMBL5571929 Ki - -
CHEMBL5570067 Ki - -
CHEMBL5563661 Ki - -
CHEMBL5565866 Ki - -
CHEMBL2078794 Ki - -