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Assay Detail

CHEMBL5550459

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of BODIPY TMR-X-labeled P11 peptide from recombinant N-terminal deca-His/SUMO tagged RTA (1 to 267 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as inhibition constant incubated for 30 mins by competition binding based fluorescence polarization assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Not specified
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Structure-based design and optimization of a new class of small molecule inhibitors targeting the P-stalk binding pocket of ricin.
Rudolph MJ, Dutta A, Tsymbal AM, McLaughlin JE, Chen Y, Davis SA, Theodorous SA, Pierce M, Algava B, Zhang X, Szekely Z, Roberge JY, Li XP, Tumer NE.
Bioorg Med Chem (2024) 100 : 117614 -117614
PubMed 38340640 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 20 4.90 6.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5570679 1 6.00
CHEMBL5564152 1 6.00
CHEMBL5569646 1 5.40
CHEMBL5081858 1 5.10
CHEMBL1421818 1 5.10
CHEMBL5583644 1 4.82
CHEMBL5583562 1 4.75
CHEMBL1499110 1 4.70
CHEMBL5573290 1 4.64
CHEMBL3758678 1 4.55
CHEMBL1567079 1 4.37
CHEMBL3951007 1 4.24
CHEMBL5573194 1 4.03
CHEMBL5573297 1 -
CHEMBL5571688 1 -
CHEMBL1373223 1 -
CHEMBL1366384 1 -
CHEMBL5571408 1 -
CHEMBL5593223 1 -
CHEMBL3758963 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5570679 Ki = 1000.0 nM 6.00
CHEMBL5564152 Ki = 1000.0 nM 6.00
CHEMBL5569646 Ki = 4000.0 nM 5.40
CHEMBL5081858 Ki = 8000.0 nM 5.10
CHEMBL1421818 Ki = 8000.0 nM 5.10
CHEMBL5583644 Ki = 15000.0 nM 4.82
CHEMBL5583562 Ki = 18000.0 nM 4.75
CHEMBL1499110 Ki = 20000.0 nM 4.70
CHEMBL5573290 Ki = 23000.0 nM 4.64
CHEMBL3758678 Ki = 28000.0 nM 4.55
CHEMBL1567079 Ki = 43000.0 nM 4.37
CHEMBL3951007 Ki = 58000.0 nM 4.24
CHEMBL5573194 Ki = 94000.0 nM 4.03
CHEMBL5573297 Ki - -
CHEMBL5571688 Ki - -
CHEMBL1373223 Ki - -
CHEMBL1366384 Ki - -
CHEMBL5571408 Ki - -
CHEMBL5593223 Ki - -
CHEMBL3758963 Ki - -