Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5554635

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human P2X3 receptor expressed in 1321N1 cells assessed as reduction in calcium level preincubated for 3 mins followed by alpha, beta-meATP addition and measured after 3 mins by Fluo-4 AM dye based microplate reader assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type 1321N1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2X purinoceptor 3 (CHEMBL2998)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Triazolopyrimidine Derivatives as Selective P2X3 Receptor Antagonists Binding to an Unprecedented Allosteric Site as Evidenced by Cryo-Electron Microscopy.
Kim GR, Kim S, Kim YO, Han X, Nagel J, Kim J, Song DI, Müller CE, Yoon MH, Jin MS, Kim YC.
J Med Chem (2024) 67 : 14443 -14465
PubMed 39102524 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 1 7.66 7.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL596234 A-317491 1 7.66

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL596234 A-317491 Ki = 22.0 nM 7.66