Assay Detail
Functional
CHEMBL5554804
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Antiproliferative activity against human HepG2 cells assessed as inhibition of cell proliferation measured after 3 days by MTT assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HepG2 |
| Confidence | 1 — Organism |
| Curated By | Autocuration |
Publication
Discovering New Metallo-Deubiquitinase CSN5 Inhibitors by a Non-Catalytic Activity Assay Platform.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 13 | 4.90 | 5.53 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL9470 | SHIKONIN | — | 1 | 5.53 |
| CHEMBL28457 | ALKANNIN | — | 1 | 5.31 |
| CHEMBL274056 | NAPHTHAZALIN | — | 1 | 5.30 |
| CHEMBL403516 | ACETYLSHIKONIN | — | 1 | 5.19 |
| CHEMBL9416 | ISOBUTYLSHIKONIN | — | 1 | 5.06 |
| CHEMBL486627 | DEOXYSHIKONIN | — | 1 | 4.98 |
| CHEMBL5566554 | — | — | 1 | 4.51 |
| CHEMBL5574229 | — | — | 1 | 4.43 |
| CHEMBL5572586 | — | — | 1 | 4.41 |
| CHEMBL5575733 | — | — | 1 | 4.28 |
| CHEMBL5569845 | — | — | 1 | - |
| CHEMBL5572812 | — | — | 1 | - |
| CHEMBL5573752 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL9470 | SHIKONIN | IC50 | = | 2960.0 | nM | 5.53 |
| CHEMBL28457 | ALKANNIN | IC50 | = | 4860.0 | nM | 5.31 |
| CHEMBL274056 | NAPHTHAZALIN | IC50 | = | 4970.0 | nM | 5.30 |
| CHEMBL403516 | ACETYLSHIKONIN | IC50 | = | 6480.0 | nM | 5.19 |
| CHEMBL9416 | ISOBUTYLSHIKONIN | IC50 | = | 8730.0 | nM | 5.06 |
| CHEMBL486627 | DEOXYSHIKONIN | IC50 | = | 10500.0 | nM | 4.98 |
| CHEMBL5566554 | — | IC50 | = | 30800.0 | nM | 4.51 |
| CHEMBL5574229 | — | IC50 | = | 37290.0 | nM | 4.43 |
| CHEMBL5572586 | — | IC50 | = | 39140.0 | nM | 4.41 |
| CHEMBL5575733 | — | IC50 | = | 52070.0 | nM | 4.28 |
| CHEMBL5569845 | — | IC50 | > | 100000.0 | nM | - |
| CHEMBL5572812 | — | IC50 | > | 100000.0 | nM | - |
| CHEMBL5573752 | — | IC50 | > | 100000.0 | nM | - |