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Assay Detail

CHEMBL5581540

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Aminopeptidase N (unknown origin) using Ala-AMC as substrate incubated for 30 mins by SpectraMax microplate reader analysis
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Aminopeptidase N (CHEMBL1907)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Modulation of endogenous opioid signaling by inhibitors of puromycin-sensitive aminopeptidase.
Singh R, Jiang R, Williams J, Dobariya P, Hanak F, Xie J, Rothwell PE, Vince R, More SS.
Eur J Med Chem (2024) 275 : 116604 -116604
PubMed 38917665 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 6.04 7.33

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5591455 1 7.33
CHEMBL5590634 1 7.08
CHEMBL3244800 1 6.38
CHEMBL2103847 TOSEDOSTAT 2.0 1 6.17
CHEMBL29292 UBENIMEX 2.0 1 6.10
CHEMBL5595970 1 5.58
CHEMBL4161892 1 5.25
CHEMBL469912 PUROMYCIN 2.0 1 4.39
CHEMBL10247 THIORPHAN -1.0 1 -
CHEMBL5590074 1 -
CHEMBL5594450 1 -
CHEMBL5591560 1 -
CHEMBL5593353 1 -
CHEMBL5594248 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5591455 IC50 = 47.0 nM 7.33
CHEMBL5590634 IC50 = 84.0 nM 7.08
CHEMBL3244800 IC50 = 420.0 nM 6.38
CHEMBL2103847 TOSEDOSTAT IC50 = 680.0 nM 6.17
CHEMBL29292 UBENIMEX IC50 = 800.0 nM 6.10
CHEMBL5595970 IC50 = 2600.0 nM 5.58
CHEMBL4161892 IC50 = 5600.0 nM 5.25
CHEMBL469912 PUROMYCIN IC50 = 41000.0 nM 4.39
CHEMBL10247 THIORPHAN IC50 > 100000.0 nM -
CHEMBL5590074 IC50 > 100000.0 nM -
CHEMBL5594450 IC50 > 100000.0 nM -
CHEMBL5591560 IC50 > 100000.0 nM -
CHEMBL5593353 IC50 > 100000.0 nM -
CHEMBL5594248 IC50 > 100000.0 nM -