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Assay Detail

CHEMBL5581972

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human Avi-tagged MUS81 (246 to 551 residues)/2x8His-tagged EME1 (246 to 570 residues) extracted from Escherichia coli BL21 Gold (DE3) using branched DNA as substrate preincubated for 15 mins followed by substrate addition and measured after 90 mins by FRET assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Fragment-Based Discovery of Novel MUS81 Inhibitors.
Collie GW, Börjesson U, Chen Y, Dong Z, Di Fruscia P, Gohlke A, Hoyle A, Hunt TA, Jesani MH, Luo H, Luptak J, Milbradt AG, Narasimhan P, Packer M, Patel S, Qiao J, Storer RI, Stubbs CJ, Tart J, Truman C, Wang AT, Wheeler MG, Winter-Holt J.
ACS Med Chem Lett (2024) 15 : 1151 -1158
PubMed 39015284 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 5.80 6.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5591377 1 6.57
CHEMBL5592380 1 6.50
CHEMBL5594391 1 6.33
CHEMBL5594260 1 6.33
CHEMBL5594006 1 6.18
CHEMBL5595635 1 5.96
CHEMBL5594490 1 5.92
CHEMBL5595627 1 5.82
CHEMBL4226761 1 5.70
CHEMBL5592602 1 5.70
CHEMBL188448 1 5.68
CHEMBL5188107 1 5.62
CHEMBL203976 1 5.55
CHEMBL5595231 1 5.55
CHEMBL5594819 1 5.42
CHEMBL440562 1 5.41
CHEMBL5593401 1 5.39
CHEMBL5592973 1 5.35
CHEMBL4225408 1 5.25
CHEMBL3617204 1 -
CHEMBL4174171 1 -
CHEMBL5595842 1 -
CHEMBL5584056 1 -
CHEMBL5595830 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5591377 IC50 = 270.0 nM 6.57
CHEMBL5592380 IC50 = 320.0 nM 6.50
CHEMBL5594391 IC50 = 470.0 nM 6.33
CHEMBL5594260 IC50 = 470.0 nM 6.33
CHEMBL5594006 IC50 = 660.0 nM 6.18
CHEMBL5595635 IC50 = 1100.0 nM 5.96
CHEMBL5594490 IC50 = 1200.0 nM 5.92
CHEMBL5595627 IC50 = 1500.0 nM 5.82
CHEMBL4226761 IC50 = 2000.0 nM 5.70
CHEMBL5592602 IC50 = 2000.0 nM 5.70
CHEMBL188448 IC50 = 2100.0 nM 5.68
CHEMBL5188107 IC50 = 2400.0 nM 5.62
CHEMBL203976 IC50 = 2800.0 nM 5.55
CHEMBL5595231 IC50 = 2800.0 nM 5.55
CHEMBL5594819 IC50 = 3800.0 nM 5.42
CHEMBL440562 IC50 = 3900.0 nM 5.41
CHEMBL5593401 IC50 = 4100.0 nM 5.39
CHEMBL5592973 IC50 = 4500.0 nM 5.35
CHEMBL4225408 IC50 = 5600.0 nM 5.25
CHEMBL3617204 IC50 > 100000.0 nM -
CHEMBL4174171 IC50 > 100000.0 nM -
CHEMBL5595842 IC50 > 100000.0 nM -
CHEMBL5584056 IC50 > 100000.0 nM -
CHEMBL5595830 IC50 > 100000.0 nM -