Assay Detail
Binding
CHEMBL5584587
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of N-terminal poly his-tagged recombinant RIPK2 (8 to 310 residues) (unknown origin) expressed in Sf9 cells preincubated for 15 mins followed by ATP addition and measured after 90 mins mins by by ADP-Glo reagent based luminescence assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Receptor-interacting serine/threonine-protein kinase 2 (CHEMBL5014) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Design, synthesis and evaluation of novel thieno[2,3d]pyrimidine derivatives as potent and specific RIPK2 inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 24 | 8.02 | 8.52 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5598306 | — | — | 1 | 8.52 |
| CHEMBL4514780 | — | — | 1 | 8.40 |
| CHEMBL5596566 | — | — | 1 | 8.40 |
| CHEMBL5596719 | — | — | 1 | 8.40 |
| CHEMBL5596475 | — | — | 1 | 8.30 |
| CHEMBL5597795 | — | — | 1 | 8.30 |
| CHEMBL5597463 | — | — | 1 | 8.15 |
| CHEMBL5598474 | — | — | 1 | 8.10 |
| CHEMBL5596401 | — | — | 1 | 8.10 |
| CHEMBL5590947 | — | — | 1 | 8.05 |
| CHEMBL5597680 | — | — | 1 | 8.00 |
| CHEMBL5598225 | — | — | 1 | 7.96 |
| CHEMBL5597383 | — | — | 1 | 7.96 |
| CHEMBL5596824 | — | — | 1 | 7.96 |
| CHEMBL5596171 | — | — | 1 | 7.92 |
| CHEMBL5598127 | — | — | 1 | 7.89 |
| CHEMBL5598037 | — | — | 1 | 7.85 |
| CHEMBL5597768 | — | — | 1 | 7.82 |
| CHEMBL5597925 | — | — | 1 | 7.80 |
| CHEMBL5598319 | — | — | 1 | 7.77 |
| CHEMBL5597496 | — | — | 1 | 7.77 |
| CHEMBL5597467 | — | — | 1 | 7.75 |
| CHEMBL5597059 | — | — | 1 | 7.68 |
| CHEMBL5597382 | — | — | 1 | 7.58 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5598306 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL4514780 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL5596566 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL5596719 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL5596475 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL5597795 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL5597463 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL5598474 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL5596401 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL5590947 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL5597680 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL5598225 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL5597383 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL5596824 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL5596171 | — | IC50 | = | 12.0 | nM | 7.92 |
| CHEMBL5598127 | — | IC50 | = | 13.0 | nM | 7.89 |
| CHEMBL5598037 | — | IC50 | = | 14.0 | nM | 7.85 |
| CHEMBL5597768 | — | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL5597925 | — | IC50 | = | 16.0 | nM | 7.80 |
| CHEMBL5598319 | — | IC50 | = | 17.0 | nM | 7.77 |
| CHEMBL5597496 | — | IC50 | = | 17.0 | nM | 7.77 |
| CHEMBL5597467 | — | IC50 | = | 18.0 | nM | 7.75 |
| CHEMBL5597059 | — | IC50 | = | 21.0 | nM | 7.68 |
| CHEMBL5597382 | — | IC50 | = | 26.0 | nM | 7.58 |