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Assay Detail

CHEMBL5584719

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human 5-HT7 receptor extracted from HEK293 cell membrane assessed as inhibition of [3H]LSD binding to 5-HT7 by measuring inhibition constant at 1 uM incubated for 1 hrs by microbeta2 reader
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 7 (CHEMBL3155)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and biological evaluation of aralkyl piperazine and piperidine derivatives targeting SSRI/5-HT<sub>1A</sub>/5-HT<sub>7</sub>.
Wu J, Zhang Z, Zhang Q, Li J.
Bioorg Med Chem (2024) 104 : 117698 -117698
PubMed 38552597 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 8.34 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL39 SEROTONIN 3.0 1 9.70
CHEMBL3401493 1 8.99
CHEMBL5597179 1 8.96
CHEMBL5598295 1 8.57
CHEMBL5597415 1 8.22
CHEMBL5597217 1 8.19
CHEMBL224820 1 8.07
CHEMBL5598106 1 7.81
CHEMBL4434876 1 7.57
CHEMBL225284 1 7.28

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL39 SEROTONIN Ki = 0.2 nM 9.70
CHEMBL3401493 Ki = 1.03 nM 8.99
CHEMBL5597179 Ki = 1.1 nM 8.96
CHEMBL5598295 Ki = 2.7 nM 8.57
CHEMBL5597415 Ki = 6.0 nM 8.22
CHEMBL5597217 Ki = 6.5 nM 8.19
CHEMBL224820 Ki = 8.6 nM 8.07
CHEMBL5598106 Ki = 15.4 nM 7.81
CHEMBL4434876 Ki = 26.8 nM 7.57
CHEMBL225284 Ki = 52.7 nM 7.28