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Assay Detail

CHEMBL5586080

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of 2-((5-(7-chloro-2-((3-((2-(5-(3-(5,5-difluoro-7,9-dimethyl-5H-4lambda4,5lambda4-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-3-yl)propanamido)pentanamido)ethyl)amino)-3-oxopropyl)amino)-4H-benzo[5,6]cyclohepta[1,2-d]thiazol-4-yl)-2-oxo-4-thioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)-N-(1H-tetrazol-5-yl)thiazole-4-carboxamide from NLuc fused P2Y2R (unknown origin) extracted from 1321N1 cell membrane incubated for 1 hr by NanoBRET assay
11
Total Activities
10
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type 1321N1
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2Y purinoceptor 2 (CHEMBL4398)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, Synthesis, and Evaluation of a New Chemotype Fluorescent Ligand for the P2Y<sub>2</sub> Receptor.
Knight R, Kilpatrick LE, Hill SJ, Stocks MJ.
ACS Med Chem Lett (2024) 15 : 1127 -1135
PubMed 39015271 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 5.30 7.49
Activity 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4082045 1 7.49
CHEMBL5597711 1 5.61
CHEMBL5596071 2 5.48
CHEMBL5596941 1 5.17
CHEMBL5596524 1 5.04
CHEMBL5591024 1 4.87
CHEMBL5598495 1 4.84
CHEMBL5597487 1 4.71
CHEMBL5596778 1 4.49
CHEMBL5597798 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4082045 Ki = 32.36 nM 7.49
CHEMBL5597711 Ki = 2454.71 nM 5.61
CHEMBL5596071 Ki = 3311.31 nM 5.48
CHEMBL5596941 Ki = 6760.83 nM 5.17
CHEMBL5596524 Ki = 9120.11 nM 5.04
CHEMBL5591024 Ki = 13489.63 nM 4.87
CHEMBL5598495 Ki = 14454.4 nM 4.84
CHEMBL5597487 Ki = 19498.45 nM 4.71
CHEMBL5596778 Ki = 32359.37 nM 4.49
CHEMBL5597798 Ki ~ 1000000.0 nM -
CHEMBL5596071 Activity - -