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Assay Detail

CHEMBL5586209

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Binding
Inhibition of wild type Plasmodium falciparum DHFR N51I/C59R/S108N/I164L quadruple mutant expressed in Escherichia coli BL21(DE3) pLysS cells assessed as inhibition constant using DHF as substrate measured for 80 sec in presence of NADPH
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Plasmodium falciparum
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Novel flexible biphenyl <i>Pf</i>DHFR inhibitors with improved antimalarial activity.
Decharuangsilp S, Arwon U, Sooksai N, Rattanajak R, Saeyang T, Vitsupakorn D, Vanichtanankul J, Yuthavong Y, Kamchonwongpaisan S, Hoarau M.
RSC Med Chem (2024) 15 : 2496 -2507
PubMed 39026651 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 9.32 9.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5598041 1 9.62
CHEMBL5597998 1 9.51
CHEMBL5596188 1 9.47
CHEMBL5597854 1 9.35
CHEMBL5597280 1 9.22
CHEMBL5597152 1 9.21
CHEMBL5598010 1 9.19
CHEMBL5590041 1 9.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5598041 Ki = 0.24 nM 9.62
CHEMBL5597998 Ki = 0.31 nM 9.51
CHEMBL5596188 Ki = 0.34 nM 9.47
CHEMBL5597854 Ki = 0.45 nM 9.35
CHEMBL5597280 Ki = 0.6 nM 9.22
CHEMBL5597152 Ki = 0.62 nM 9.21
CHEMBL5598010 Ki = 0.64 nM 9.19
CHEMBL5590041 Ki = 0.99 nM 9.00