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Assay Detail

CHEMBL5588085

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiproliferative activity against mouse C26 cells harboring KRAS G12D mutant incubated for 72 hrs by CCK8 assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type C-26
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Design, Synthesis, and Biological Evaluation of 5-Amino-4-fluoro-1<i>H</i>-benzo[<i>d</i>]imidazole-6-carboxamide Derivatives as Novel and Potential MEK/RAF Complex Inhibitors Based on the "Clamp" Strategy.
Wang P, Yuan Y, Yang T, Zou Y, Tang M, Ma Z, Bo W, Qin S, Chen Y, Guo T, Guo Z, Yang J, Xiang M, Chen L.
J Med Chem (2024) 67 : 15246 -15267
PubMed 39166848 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 7.14 7.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5596553 1 7.96
CHEMBL5597799 1 7.25
CHEMBL5597316 1 7.02
CHEMBL5597586 1 6.82
CHEMBL5596257 1 6.65

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5596553 IC50 = 11.0 nM 7.96
CHEMBL5597799 IC50 = 56.0 nM 7.25
CHEMBL5597316 IC50 = 96.0 nM 7.02
CHEMBL5597586 IC50 = 152.0 nM 6.82
CHEMBL5596257 IC50 = 226.0 nM 6.65