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Assay Detail

CHEMBL5611200

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal GST tagged full length human recombinant HDAC6 (1 to 1215 residues) extracted from Sf9 cells using Z-Lys(Ac)-AMC as substrate measured after 90 mins by fluorescence based microplate reader analysis
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 6 (CHEMBL1865)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Contilisant+Tubastatin A Hybrids: Polyfunctionalized Indole Derivatives as New HDAC Inhibitor-Based Multitarget Small Molecules with <i>In Vitro</i> and <i>In Vivo</i> Activity in Neurodegenerative Diseases.
Toledano-Pinedo M, Porro-Pérez A, Schäker-Hübner L, Romero F, Dong M, Samadi A, Almendros P, Iriepa I, Bautista-Aguilera ÒM, Rodríguez-Fernández MM, Solana-Manrique C, Sanchis I, Mora-Morell A, Rodrìguez AC, Sànchez-Pérez AM, Knez D, Gobec S, Bellver-Sanchis A, Pérez B, Dobrydnev AV, Artetxe-Zurutuza A, Matheu A, Siwek A, Wolak M, Satała G, Bojarski AJ, Doroz-Płonka A, Handzlik J, Godyń J, Więckowska A, Paricio N, Griñán-Ferré C, Hansen FK, Marco-Contelles J.
J Med Chem (2024) 67 : 16533 -16555
PubMed 39256214 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.34 7.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5612392 1 7.92
CHEMBL2018302 TUBASTATIN A 1 7.80
CHEMBL98 VORINOSTAT 4.0 1 7.54
CHEMBL5612289 1 7.46
CHEMBL5611957 1 6.78
CHEMBL5612800 1 6.53
CHEMBL5613926 1 -
CHEMBL4173258 1 -
CHEMBL5614106 1 -
CHEMBL5612700 1 -
CHEMBL5614345 1 -
CHEMBL5613087 1 -
CHEMBL5613964 1 -
CHEMBL5612956 1 -
CHEMBL5613368 1 -
CHEMBL5614364 1 -
CHEMBL5613800 1 -
CHEMBL5613037 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5612392 IC50 = 12.0 nM 7.92
CHEMBL2018302 TUBASTATIN A IC50 = 16.0 nM 7.80
CHEMBL98 VORINOSTAT IC50 = 29.0 nM 7.54
CHEMBL5612289 IC50 = 35.0 nM 7.46
CHEMBL5611957 IC50 = 168.0 nM 6.78
CHEMBL5612800 IC50 = 295.0 nM 6.53
CHEMBL5613926 IC50 > 10000.0 nM -
CHEMBL4173258 IC50 > 10000.0 nM -
CHEMBL5614106 IC50 > 10000.0 nM -
CHEMBL5612700 IC50 > 3333.0 nM -
CHEMBL5614345 IC50 > 10000.0 nM -
CHEMBL5613087 IC50 > 10000.0 nM -
CHEMBL5613964 IC50 > 10000.0 nM -
CHEMBL5612956 IC50 > 10000.0 nM -
CHEMBL5613368 IC50 > 10000.0 nM -
CHEMBL5614364 IC50 > 10000.0 nM -
CHEMBL5613800 IC50 > 10000.0 nM -
CHEMBL5613037 IC50 > 10000.0 nM -