Assay Detail
Binding
CHEMBL5615239
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of biotinylated Fc-tagged ephrin-A1 from recombinant N/C-terminal 6-his tagged mouse EphA8 (Gly27 to Arg540 residues) preincubated for 1 hr followed by ephrin-A1-Fc addition and measured after 4 to 5 hrs by ELISA assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Discovery of a new 1-(phenylsulfonyl)-1H-indole derivative targeting the EphA2 receptor with antiproliferative activity on U251 glioblastoma cell line.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 5.30 | 5.75 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3735992 | — | — | 1 | 5.75 |
| CHEMBL5619796 | — | — | 1 | 5.26 |
| CHEMBL5619189 | — | — | 1 | 4.89 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3735992 | — | IC50 | = | 1800.0 | nM | 5.75 |
| CHEMBL5619796 | — | IC50 | = | 5500.0 | nM | 5.26 |
| CHEMBL5619189 | — | IC50 | = | 13000.0 | nM | 4.89 |