Assay Detail
Binding
CHEMBL5615243
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of biotinylated Fc-tagged ephrin-A1 from C/N-terminal 6-His tag mouse recombinant EphA2 (Ala22 to Ala535 residues) assessed as inhibition constant preincubated for 1 hr followed by ephrin-A1-Fc addition and measured after 4 to 5 hrs by ELISA assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Discovery of a new 1-(phenylsulfonyl)-1H-indole derivative targeting the EphA2 receptor with antiproliferative activity on U251 glioblastoma cell line.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 3 | 5.69 | 5.85 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3735992 | — | — | 1 | 5.85 |
| CHEMBL5619796 | — | — | 1 | 5.66 |
| CHEMBL5619189 | — | — | 1 | 5.55 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3735992 | — | Ki | = | 1400.0 | nM | 5.85 |
| CHEMBL5619796 | — | Ki | = | 2200.0 | nM | 5.66 |
| CHEMBL5619189 | — | Ki | = | 2800.0 | nM | 5.55 |