Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5617873

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Allosteric inhibition of N-terminal His-tagged human recombinant SHP2 extracted from Escherichia coli using DiFMUP as substrate preincubated with compound for 60 mins followed by substrate addition and measured after 30 mins by fluorescence based microplate reader analysis
44
Total Activities
44
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein phosphatase non-receptor type 11 (CHEMBL3864)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of novel substituted pyridine carboxamide derivatives as potent allosteric SHP2 inhibitors.
Lv X, Li P, Chen Z, Huang S, Zhang S, Ji B, Liu J, Du T, Zhang T, Chen X, Qiang L, He Y, Lai Y.
Eur J Med Chem (2024) 279 : 116830 -116830
PubMed 39303516 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 44 7.66 9.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5618343 1 9.89
CHEMBL5618733 1 9.89
CHEMBL5619476 1 9.77
CHEMBL5619863 1 9.23
CHEMBL5619365 1 9.22
CHEMBL5619074 1 8.99
CHEMBL5619866 1 8.97
CHEMBL5618566 1 8.77
CHEMBL5620154 1 8.73
CHEMBL5619937 1 8.19
CHEMBL5618608 1 8.18
CHEMBL5619118 1 7.95
CHEMBL4650521 BATOPROTAFIB 2.0 1 7.70
CHEMBL5619482 1 7.53
CHEMBL5619265 1 7.52
CHEMBL5618322 1 7.49
CHEMBL5619941 1 7.44
CHEMBL5619774 1 7.40
CHEMBL5620322 1 7.30
CHEMBL5618116 1 7.29
CHEMBL5619759 1 7.28
CHEMBL5620194 1 7.17
CHEMBL5618941 1 7.11
CHEMBL5618632 1 7.05
CHEMBL5618351 1 7.05
CHEMBL5618981 1 6.95
CHEMBL5620018 1 6.93
CHEMBL5619709 1 6.89
CHEMBL5620236 1 6.83
CHEMBL5619832 1 6.76

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5618343 IC50 = 0.13 nM 9.89
CHEMBL5618733 IC50 = 0.13 nM 9.89
CHEMBL5619476 IC50 = 0.17 nM 9.77
CHEMBL5619863 IC50 = 0.59 nM 9.23
CHEMBL5619365 IC50 = 0.6 nM 9.22
CHEMBL5619074 IC50 = 1.03 nM 8.99
CHEMBL5619866 IC50 = 1.08 nM 8.97
CHEMBL5618566 IC50 = 1.7 nM 8.77
CHEMBL5620154 IC50 = 1.87 nM 8.73
CHEMBL5619937 IC50 = 6.4 nM 8.19
CHEMBL5618608 IC50 = 6.6 nM 8.18
CHEMBL5619118 IC50 = 11.3 nM 7.95
CHEMBL4650521 BATOPROTAFIB IC50 = 20.1 nM 7.70
CHEMBL5619482 IC50 = 29.4 nM 7.53
CHEMBL5619265 IC50 = 30.1 nM 7.52
CHEMBL5618322 IC50 = 32.3 nM 7.49
CHEMBL5619941 IC50 = 36.2 nM 7.44
CHEMBL5619774 IC50 = 39.6 nM 7.40
CHEMBL5620322 IC50 = 49.8 nM 7.30
CHEMBL5618116 IC50 = 51.9 nM 7.29
CHEMBL5619759 IC50 = 52.8 nM 7.28
CHEMBL5620194 IC50 = 67.8 nM 7.17
CHEMBL5618941 IC50 = 77.0 nM 7.11
CHEMBL5618351 IC50 = 89.1 nM 7.05
CHEMBL5618632 IC50 = 88.6 nM 7.05
CHEMBL5618981 IC50 = 113.1 nM 6.95
CHEMBL5620018 IC50 = 118.6 nM 6.93
CHEMBL5619709 IC50 = 127.9 nM 6.89
CHEMBL5620236 IC50 = 146.8 nM 6.83
CHEMBL5619832 IC50 = 173.8 nM 6.76
CHEMBL5619179 IC50 = 174.5 nM 6.76
CHEMBL5619285 IC50 = 217.6 nM 6.66
CHEMBL5619949 IC50 = 219.3 nM 6.66
CHEMBL5618829 IC50 = 296.3 nM 6.53
CHEMBL5620279 IC50 = 332.2 nM 6.48
CHEMBL5619377 IC50 = 364.8 nM 6.44
CHEMBL5618125 IC50 = 530.0 nM 6.28
CHEMBL5618370 IC50 - -
CHEMBL5618614 IC50 - -
CHEMBL5618947 IC50 - -
CHEMBL5618993 IC50 - -
CHEMBL5618658 IC50 - -
CHEMBL5618236 IC50 - -
CHEMBL5619628 IC50 - -