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Assay Detail

CHEMBL5625323

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length N-terminal GST-tagged human IDH1 R132H mutant expressed in Escherichia coli in presence of alpha-KG by fluorescence based biochemical assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Isocitrate dehydrogenase [NADP] cytoplasmic (CHEMBL2007625)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Challenges for the development of mutant isocitrate dehydrogenases 1 inhibitors to treat glioma.
Wang QX, Zhang PY, Li QQ, Tong ZJ, Wu JZ, Yu SP, Yu YC, Ding N, Leng XJ, Chang L, Xu JG, Sun SL, Yang Y, Li NG, Shi ZH.
Eur J Med Chem (2023) 257 : 115464 -115464
PubMed 37235998 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 6.16 7.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5631891 1 7.30
CHEMBL5632522 1 6.80
CHEMBL5632442 1 5.81
CHEMBL5632408 1 4.71

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5631891 IC50 = 50.0 nM 7.30
CHEMBL5632522 IC50 = 160.0 nM 6.80
CHEMBL5632442 IC50 = 1540.0 nM 5.81
CHEMBL5632408 IC50 = 19390.0 nM 4.71