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Assay Detail

CHEMBL5625328

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human wild type IDH1 R132H mutant expressed in Escherichia coli BL21 (DE3) cells incubated for 60 mins by fluorescence based analysis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Isocitrate dehydrogenase [NADP] cytoplasmic (CHEMBL2007625)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Challenges for the development of mutant isocitrate dehydrogenases 1 inhibitors to treat glioma.
Wang QX, Zhang PY, Li QQ, Tong ZJ, Wu JZ, Yu SP, Yu YC, Ding N, Leng XJ, Chang L, Xu JG, Sun SL, Yang Y, Li NG, Shi ZH.
Eur J Med Chem (2023) 257 : 115464 -115464
PubMed 37235998 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 8.35 9.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5633097 1 9.46
CHEMBL5633056 1 8.22
CHEMBL5633305 1 8.22
CHEMBL4651002 SAFUSIDENIB 2.0 1 8.08
CHEMBL5632905 1 7.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5633097 IC50 = 0.349 nM 9.46
CHEMBL5633056 IC50 = 6.0 nM 8.22
CHEMBL5633305 IC50 = 6.0 nM 8.22
CHEMBL4651002 SAFUSIDENIB IC50 = 8.4 nM 8.08
CHEMBL5632905 IC50 = 18.0 nM 7.75