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Assay Detail

CHEMBL5634919

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Anti-adipogenic activity in mouse 3T3-L1 cells assessed as lipid-lowering activity measured after 9 days by Oil Red O staining based microplate reader analysis
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type 3T3-L1
Confidence 1 — Organism
Curated By Autocuration

Target

3T3-L1 (CHEMBL614510)
Type CELL-LINE
Organism Mus musculus

Publication

Design, synthesis and evaluation of 2-pyrimidinylindole derivatives as anti-obesity agents by regulating lipid metabolism.
Guo SY, Wei LY, Song BB, Hu YT, Jiang Z, Zhao DD, Xu YH, Lin YW, Xu SM, Chen SB, Huang ZS.
Eur J Med Chem (2023) 260 : 115729 -115729
PubMed 37607439 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 13 6.82 7.35

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5639170 1 7.35
CHEMBL5402446 1 7.11
CHEMBL5639890 1 7.05
CHEMBL301982 CELASTROL 1 7.00
CHEMBL5647575 1 6.97
CHEMBL5641669 1 6.96
CHEMBL5639940 1 6.90
CHEMBL5639250 1 6.75
CHEMBL5641657 1 6.65
CHEMBL5646707 1 6.59
CHEMBL5647509 1 6.53
CHEMBL5647491 1 6.51
CHEMBL5646393 1 6.29

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5639170 EC50 = 45.0 nM 7.35
CHEMBL5402446 EC50 = 78.0 nM 7.11
CHEMBL5639890 EC50 = 89.0 nM 7.05
CHEMBL301982 CELASTROL EC50 = 99.0 nM 7.00
CHEMBL5647575 EC50 = 106.0 nM 6.97
CHEMBL5641669 EC50 = 111.0 nM 6.96
CHEMBL5639940 EC50 = 127.0 nM 6.90
CHEMBL5639250 EC50 = 180.0 nM 6.75
CHEMBL5641657 EC50 = 225.0 nM 6.65
CHEMBL5646707 EC50 = 254.0 nM 6.59
CHEMBL5647509 EC50 = 295.0 nM 6.53
CHEMBL5647491 EC50 = 309.0 nM 6.51
CHEMBL5646393 EC50 = 509.0 nM 6.29