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Assay Detail

CHEMBL5636913

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EZH2 Y641N mutant (unknown origin) using [3H]-SAM as substrate preincubated for 15 mins followed by substrate addition and measured after 180 mins by AlphaLISA assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone-lysine N-methyltransferase EZH2 (CHEMBL2189110)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of dihydropyridinone derivative as a covalent EZH2 degrader.
Zhou B, Wang B, Zou F, Mei H, Liu Q, Qi S, Wang W, Jin R, Wang A, Chen Y, Liu F, Wang W, Liu J, Liu Q.
Eur J Med Chem (2023) 261 : 115825 -115825
PubMed 37826933 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 8.72 8.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5641699 1 8.77
CHEMBL3414621 TAZEMETOSTAT 4.0 1 8.66

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5641699 IC50 = 1.7 nM 8.77
CHEMBL3414621 TAZEMETOSTAT IC50 = 2.2 nM 8.66