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Assay Detail

CHEMBL5648058

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of KDM6B (unknown origin) using biotinylated H3K27me3 peptide as substrate preincubated for 15 mins followed by substrate addition measured after 5 mins in presence of 2-oxoglutarate/Fe2+/L-ascorbic acid by AlphaScreen assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysine-specific demethylase 6B (CHEMBL1938211)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Inhibition of demethylases by GSK-J1/J4.
Heinemann B, Nielsen JM, Hudlebusch HR, Lees MJ, Larsen DV, Boesen T, Labelle M, Gerlach LO, Birk P, Helin K.
Nature (2014) 514 : 1 -2
PubMed 25279926 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.22 7.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3188597 1 7.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3188597 IC50 = 60.0 nM 7.22