Assay Detail
Binding
CHEMBL5649145
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of FGFR1 autophosphorylation in mouse NIH/3T3 cells assessed as reduction in aFGF and heparin-induced autophosphorylation incubated for 5 mins before stimulation for 5 mins with aFGF and heparin by SDS-PAGE and immunoblot method
2
Total Activities
1
Compounds Tested
2
Activity Types
6
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Cell Type | NIH/3T3 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Crystal structure of an angiogenesis inhibitor bound to the FGF receptor tyrosine kinase domain.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 9.00 | 9.00 |
| Inhibition | 1 | - | - |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| EFO_ID | EFO_ID | - | — | MONDO:0004992 |
| EFO_ID | EFO_ID | - | — | EFO:0003770 |
| EFO_ID | EFO_ID | - | — | EFO:0003914 |
| EFO_TERM | EFO_TERM | - | — | cancer |
| EFO_TERM | EFO_TERM | - | — | diabetic retinopathy |
| EFO_TERM | EFO_TERM | - | — | atherosclerosis |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL189584 | PD-173074 | — | 2 | 9.00 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL189584 | PD-173074 | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL189584 | PD-173074 | Inhibition | - | % | - |