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Assay Detail

CHEMBL5650360

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of p300 HAT domain (unknown origin) using Biotin-C6-GRGKGGKGLGKGGAK as substrate pretreated with enzyme for 30 mins followed by incubation with substrate for 1 hr by radiometric scintillation proximity assay
9
Total Activities
9
Compounds Tested
1
Activity Types
4
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone acetyltransferase p300 (CHEMBL3784)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a selective catalytic p300/CBP inhibitor that targets lineage-specific tumours.
Lasko LM, Jakob CG, Edalji RP, Qiu W, Montgomery D, Digiammarino EL, Hansen TM, Risi RM, Frey R, Manaves V, Shaw B, Algire M, Hessler P, Lam LT, Uziel T, Faivre E, Ferguson D, Buchanan FG, Martin RL, Torrent M, Chiang GG, Karukurichi K, Langston JW, Weinert BT, Choudhary C, de Vries P, Van Drie JH, McElligott D, Kesicki E, Marmorstein R, Sun C, Cole PA, Rosenberg SH, Michaelides MR, Lai A, Bromberg KD.
Nature (2017) 550 : 128 -132
PubMed 28953875 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.99 7.51

Assay Parameters

Parameter Type Value Units Text
EFO_ID EFO_ID - MONDO:0004992
EFO_ID EFO_ID - MONDO:0021259
EFO_TERM EFO_TERM - cancer
EFO_TERM EFO_TERM - prostate neoplasm

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4788713 1 7.51
CHEMBL505121 1 7.30
CHEMBL4282264 A-485 1 7.22
CHEMBL1797936 1 5.80
CHEMBL445599 1 5.30
CHEMBL4159813 1 5.29
CHEMBL5653591 1 4.94
CHEMBL140 CURCUMIN 3.0 1 4.52
CHEMBL4536197 A-486 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4788713 IC50 = 31.0 nM 7.51
CHEMBL505121 IC50 = 50.0 nM 7.30
CHEMBL4282264 A-485 IC50 = 60.0 nM 7.22
CHEMBL1797936 IC50 = 1600.0 nM 5.80
CHEMBL445599 IC50 = 5000.0 nM 5.30
CHEMBL4159813 IC50 = 5100.0 nM 5.29
CHEMBL5653591 IC50 = 11500.0 nM 4.94
CHEMBL140 CURCUMIN IC50 = 30000.0 nM 4.52
CHEMBL4536197 A-486 IC50 > 50000.0 nM -