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Assay Detail

CHEMBL5651348

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human EIF4B incubated for 45 mins by Kinobead based pull down assay
4
Total Activities
2
Compounds Tested
2
Activity Types
2
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Eukaryotic translation initiation factor 4B (CHEMBL3308928)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family.
Tröster A, Heinzlmeir S, Berger BT, Gande SL, Saxena K, Sreeramulu S, Linhard V, Nasiri AH, Bolte M, Müller S, Kuster B, Médard G, Kudlinzki D, Schwalbe H.
ChemMedChem (2018) 13 : 1629 -1633
PubMed 29928781 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 2 8.18 10.00
ED50 2 8.18 10.00

Assay Parameters

Parameter Type Value Units Text
EFO_ID EFO_ID - MONDO:0004992
EFO_TERM EFO_TERM - cancer

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5653589 2 10.00
CHEMBL3752910 2 6.35

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5653589 Kd = 0.1 nM 10.00
CHEMBL5653589 ED50 = 0.1 nM 10.00
CHEMBL3752910 Kd = 449.04 nM 6.35
CHEMBL3752910 ED50 = 449.04 nM 6.35