Assay Detail
Binding
CHEMBL5653936
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human PKCbeta2 expressed in baculovirus infected Sf9 insect cells in presence of [p32-gamma]-ATP measured after 10 mins by scintillation counter analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
4
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 7.80 | 7.80 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| EFO_ID | EFO_ID | - | — | EFO:0000365 |
| EFO_ID | EFO_ID | - | — | MONDO:0018177 |
| EFO_TERM | EFO_TERM | - | — | colorectal adenocarcinoma |
| EFO_TERM | EFO_TERM | - | — | glioblastoma |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL7463 | GF-109203 | — | 1 | 7.80 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL7463 | GF-109203 | IC50 | = | 16.0 | nM | 7.80 |