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Assay Detail

CHEMBL5657598

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat neutrophil elastase using MeOSuc-AAPV-MCA as substrate measured at 60 mins interval for up to several hrs by fluorescence assay
10
Total Activities
10
Compounds Tested
1
Activity Types
2
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Freezing the Bioactive Conformation to Boost Potency: The Identification of BAY 85-8501, a Selective and Potent Inhibitor of Human Neutrophil Elastase for Pulmonary Diseases.
von Nussbaum F, Li VM, Allerheiligen S, Anlauf S, Bärfacker L, Bechem M, Delbeck M, Fitzgerald MF, Gerisch M, Gielen-Haertwig H, Haning H, Karthaus D, Lang D, Lustig K, Meibom D, Mittendorf J, Rosentreter U, Schäfer M, Schäfer S, Schamberger J, Telan LA, Tersteegen A.
ChemMedChem (2015) 10 : 1163 -1173
PubMed 26083237 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 6.88 7.82

Assay Parameters

Parameter Type Value Units Text
EFO_ID EFO_ID - EFO:0003818
EFO_TERM EFO_TERM - lung disease

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5661928 1 7.82
CHEMBL5661863 1 7.37
CHEMBL3617969 1 7.36
CHEMBL5661868 1 7.31
CHEMBL5661846 1 6.90
CHEMBL5661864 1 6.72
CHEMBL5661882 1 6.63
CHEMBL1738716 1 6.56
CHEMBL3617968 BAY-678 1 6.10
CHEMBL5661856 1 5.99

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5661928 IC50 = 15.0 nM 7.82
CHEMBL5661863 IC50 = 43.0 nM 7.37
CHEMBL3617969 IC50 = 44.0 nM 7.36
CHEMBL5661868 IC50 = 49.0 nM 7.31
CHEMBL5661846 IC50 = 126.0 nM 6.90
CHEMBL5661864 IC50 = 192.0 nM 6.72
CHEMBL5661882 IC50 = 233.0 nM 6.63
CHEMBL1738716 IC50 = 276.0 nM 6.56
CHEMBL3617968 BAY-678 IC50 = 800.0 nM 6.10
CHEMBL5661856 IC50 = 1018.0 nM 5.99