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Assay Detail

CHEMBL5658133

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human GLUT3 expressed in human GLUT1-/- DLD-1 cells assessed as reduction in glucose uptake by measuring decrease in ATP production incubated for 15 mins in presence of oxidative phosphorylation inhibitor rotenone by CellTiter-Glo Luminescent Cell Viability Assay
62
Total Activities
62
Compounds Tested
1
Activity Types
2
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type DLD-1
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Identification and Optimization of the First Highly Selective GLUT1 Inhibitor BAY-876.
Siebeneicher H, Cleve A, Rehwinkel H, Neuhaus R, Heisler I, Müller T, Bauser M, Buchmann B.
ChemMedChem (2016) 11 : 2261 -2271
PubMed 27552707 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 62 5.46 7.46

Assay Parameters

Parameter Type Value Units Text
EFO_ID EFO_ID - MONDO:0004992
EFO_TERM EFO_TERM - cancer

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5661838 1 7.46
CHEMBL5661939 1 7.07
CHEMBL5661873 1 6.96
CHEMBL411729 CYTOCHALASIN B 1 6.92
CHEMBL5661843 1 6.64
CHEMBL5661849 1 6.54
CHEMBL5661845 1 6.52
CHEMBL5661918 1 6.34
CHEMBL5661842 1 6.33
CHEMBL5661848 1 6.26
CHEMBL5661901 1 6.24
CHEMBL5661921 1 6.21
CHEMBL5661888 1 6.12
CHEMBL5661852 1 5.96
CHEMBL5661874 1 5.92
CHEMBL5661844 1 5.81
CHEMBL4448899 BAY-876 1 5.78
CHEMBL5661915 1 5.78
CHEMBL5661885 1 5.76
CHEMBL5661862 1 5.70
CHEMBL5661828 1 5.69
CHEMBL5661924 1 5.62
CHEMBL5661833 1 5.61
CHEMBL5661896 1 5.56
CHEMBL5661834 1 5.54
CHEMBL5661934 1 5.54
CHEMBL5661847 1 5.54
CHEMBL5661913 1 5.54
CHEMBL5661919 1 5.54
CHEMBL5661929 1 5.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5661838 IC50 = 35.0 nM 7.46
CHEMBL5661939 IC50 = 86.0 nM 7.07
CHEMBL5661873 IC50 = 110.0 nM 6.96
CHEMBL411729 CYTOCHALASIN B IC50 = 120.0 nM 6.92
CHEMBL5661843 IC50 = 230.0 nM 6.64
CHEMBL5661849 IC50 = 290.0 nM 6.54
CHEMBL5661845 IC50 = 300.0 nM 6.52
CHEMBL5661918 IC50 = 460.0 nM 6.34
CHEMBL5661842 IC50 = 470.0 nM 6.33
CHEMBL5661848 IC50 = 550.0 nM 6.26
CHEMBL5661901 IC50 = 580.0 nM 6.24
CHEMBL5661921 IC50 = 610.0 nM 6.21
CHEMBL5661888 IC50 = 760.0 nM 6.12
CHEMBL5661852 IC50 = 1110.0 nM 5.96
CHEMBL5661874 IC50 = 1190.0 nM 5.92
CHEMBL5661844 IC50 = 1550.0 nM 5.81
CHEMBL4448899 BAY-876 IC50 = 1670.0 nM 5.78
CHEMBL5661915 IC50 = 1670.0 nM 5.78
CHEMBL5661885 IC50 = 1720.0 nM 5.76
CHEMBL5661862 IC50 = 1990.0 nM 5.70
CHEMBL5661828 IC50 = 2030.0 nM 5.69
CHEMBL5661924 IC50 = 2390.0 nM 5.62
CHEMBL5661833 IC50 = 2480.0 nM 5.61
CHEMBL5661896 IC50 = 2740.0 nM 5.56
CHEMBL5661919 IC50 = 2860.0 nM 5.54
CHEMBL5661934 IC50 = 2920.0 nM 5.54
CHEMBL5661913 IC50 = 2880.0 nM 5.54
CHEMBL5661834 IC50 = 2890.0 nM 5.54
CHEMBL5661847 IC50 = 2850.0 nM 5.54
CHEMBL5661929 IC50 = 3150.0 nM 5.50
CHEMBL5661908 IC50 = 3270.0 nM 5.49
CHEMBL5661884 IC50 = 3820.0 nM 5.42
CHEMBL5661895 IC50 = 4320.0 nM 5.37
CHEMBL5661861 IC50 = 4980.0 nM 5.30
CHEMBL5661920 IC50 = 5020.0 nM 5.30
CHEMBL4552180 BAY-588 IC50 = 5470.0 nM 5.26
CHEMBL5661853 IC50 = 5650.0 nM 5.25
CHEMBL5661878 IC50 = 5950.0 nM 5.22
CHEMBL5661910 IC50 = 6070.0 nM 5.22
CHEMBL5661942 IC50 = 6090.0 nM 5.21
CHEMBL5661850 IC50 = 6550.0 nM 5.18
CHEMBL5661911 IC50 = 7960.0 nM 5.10
CHEMBL5661841 IC50 = 7960.0 nM 5.10
CHEMBL5661867 IC50 = 9090.0 nM 5.04
CHEMBL5661890 IC50 = 9890.0 nM 5.00
CHEMBL5661840 IC50 = 10100.0 nM 5.00
CHEMBL5661892 IC50 = 10300.0 nM 4.99
CHEMBL5661865 IC50 = 10400.0 nM 4.98
CHEMBL5661907 IC50 = 14700.0 nM 4.83
CHEMBL5661925 IC50 = 15600.0 nM 4.81