Assay Detail
Binding
CHEMBL5659651
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of ULK1 phosphorylation at Ser15 residue in HEK293 cells incubated for 1 hr by chemiluminescence based immunoblotting analysis
6
Total Activities
3
Compounds Tested
2
Activity Types
2
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293T |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Serine/threonine-protein kinase ULK1 (CHEMBL6006) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
A Potent and Selective ULK1 Inhibitor Suppresses Autophagy and Sensitizes Cancer Cells to Nutrient Stress.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 3 | 6.37 | 7.08 |
| Inhibition | 3 | - | - |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| EFO_ID | EFO_ID | - | — | MONDO:0004992 |
| EFO_TERM | EFO_TERM | - | — | cancer |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5568152 | — | — | 2 | 7.08 |
| CHEMBL4744680 | — | — | 2 | 6.41 |
| CHEMBL4128069 | — | — | 2 | 5.62 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5568152 | — | EC50 | = | 83.0 | nM | 7.08 |
| CHEMBL4744680 | — | EC50 | = | 390.0 | nM | 6.41 |
| CHEMBL4128069 | — | EC50 | = | 2400.0 | nM | 5.62 |
| CHEMBL5568152 | — | Inhibition | - | % | - | |
| CHEMBL4744680 | — | Inhibition | - | % | - | |
| CHEMBL4128069 | — | Inhibition | - | % | - |