Assay Detail
Functional
CHEMBL5665149
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Positive allosteric modulator activity at GPR68 expressed in human HEK293T cells assessed as proton-mediated calcium release at 10 uM incubated for 10 mins by Fluo-4 Direct calcium dye based FLIPR assay (Rvb = 6.78 +/- 0.02 No_unit)
3
Total Activities
3
Compounds Tested
1
Activity Types
2
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HEK293T |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Ovarian cancer G-protein coupled receptor 1 (CHEMBL3713916) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Allosteric ligands for the pharmacologically dark receptors GPR68 and GPR65.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 3 | 6.87 | 6.94 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| EFO_ID | EFO_ID | - | — | EFO:0009386 |
| EFO_TERM | EFO_TERM | - | — | central nervous system disease |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5665399 | — | — | 1 | 6.94 |
| CHEMBL4460070 | ZINC32547799 | — | 1 | 6.85 |
| CHEMBL4548689 | Ogerin | — | 1 | 6.83 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5665399 | — | EC50 | = | 114.82 | nM | 6.94 |
| CHEMBL4460070 | ZINC32547799 | EC50 | = | 141.25 | nM | 6.85 |
| CHEMBL4548689 | Ogerin | EC50 | = | 147.91 | nM | 6.83 |