Assay Detail

CHEMBL5679156

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding

Inhibition of recombinant human GST tagged Ckh1 phosphorylation expressed in baculovirus Insect cell using N-biotinylaminohexanoyl-KKVSRSGLYRSPMPENLNRPR as peptide substrate incubated for 2 hrs in presence of ATP by TopCount scintillation proximity assay

ChEMBL ↗

Free Research Context This assay will appear in recent viewed items on this browser. Open the demo workspace to preview project-only review queues.

Research home Demo workspace

Target

Serine/threonine-protein kinase Chk1 (CHEMBL4630) ↗

Open DB target context

Use one target anchor to keep assay intent, target linkage, and tested compound review aligned.

Loading context

Serine/threonine-protein kinase Chk1 (CHEMBL4630) is the working anchor for this assay review. Loading the live Open DB target snapshot…

Primary target Serine/threonine-protein kinase Chk1

CHEMBL4630

Anchored from Assay target.

Protein identity Serine/threonine-protein kinase Chk1

Disease frame Disease evidence pending

Open the live target snapshot or evidence card before reviewing assay metadata so target fit and disease framing stay reusable.

Review focus Open DB context pending

Open the live target snapshot before relying on this target frame across surface handoff.

Open target snapshot Open evidence card Search with this context

Total Activities

Compounds Tested

Activity Types

Assay Parameters

Assay Information

Assay Type	Binding
Organism	Homo sapiens
Cell Type	Insect cell
Confidence	9 — Direct single protein target
Curated By	Autocuration

Target

Serine/threonine-protein kinase Chk1 (CHEMBL4630) ↗

Type	SINGLE PROTEIN
Organism	Homo sapiens

Publication

AZD7762, a novel checkpoint kinase inhibitor, drives checkpoint abrogation and potentiates DNA-targeted therapies.

Zabludoff SD, Deng C, Grondine MR, Sheehy AM, Ashwell S, Caleb BL, Green S, Haye HR, Horn CL, Janetka JW, Liu D, Mouchet E, Ready S, Rosenthal JL, Queva C, Schwartz GK, Taylor KJ, Tse AN, Walker GE, White AM.
Mol Cancer Ther (2008) 7 : 2955 -2966
PubMed 18790776 · DOI

Activity Statistics

Type	Count	Avg pChEMBL	Best pChEMBL
IC50	1	8.30	8.30

Assay Parameters

Parameter	Type	Value	Units	Text
EFO_ID	EFO_ID	-	—	MONDO:0004992
EFO_TERM	EFO_TERM	-	—	cancer

Compounds Tested

Compound	Name	Phase	Activities	Best pChEMBL
CHEMBL2041933	AZD-7762	1.0	1	8.30

Activity Data

Compound	Name	Type	Rel.	Value	Units	pChEMBL
CHEMBL2041933	AZD-7762	IC50	=	5.0	nM	8.30