Assay Detail
Binding
CHEMBL5680879
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Antagonist activity at mouse TRPA1 expressed in HEK293F cells assessed as reduction in AITC-induced increase in intracellular Ca2+ level by FLIPR-based Ca2+ assay
1
Total Activities
1
Compounds Tested
1
Activity Types
6
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Cell Type | HEK293F |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Transient receptor potential cation channel subfamily A member 1 (CHEMBL1075310) ↗| Type | SINGLE PROTEIN |
| Organism | Mus musculus |
Publication
Selective blockade of TRPA1 channel attenuates pathological pain without altering noxious cold sensation or body temperature regulation.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 6.53 | 6.53 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| EFO_ID | EFO_ID | - | — | HP:0012531 |
| EFO_ID | EFO_ID | - | — | EFO:1001905 |
| EFO_ID | EFO_ID | - | — | MP:0001845 |
| EFO_TERM | EFO_TERM | - | — | pain |
| EFO_TERM | EFO_TERM | - | — | oxidative stress |
| EFO_TERM | EFO_TERM | - | — | inflammation |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3697701 | A-079 | — | 1 | 6.53 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3697701 | A-079 | IC50 | = | 296.0 | nM | 6.53 |