Assay Detail
Binding
CHEMBL5684064
Review assay metadata, readout intent, target linkage, and publication context from the same page.
ATP competitive inhibition of recombinant human N-terminal GST-fused CDK4 (S4 to E303 residues)/cyclin D1 expressed in Sf9 insect cells using C-terminal Rb fragment as substrate measured after 50 mins in presence of varying levels of [33P]gamma-ATP by Michaelis-Menten equation based kinetic analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
2
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Target
Cyclin-dependent kinase 4/cyclin D1 (CHEMBL1907601) ↗| Type | PROTEIN COMPLEX |
| Organism | Homo sapiens |
Publication
Preclinical characterization of the CDK4/6 inhibitor LY2835219: in-vivo cell cycle-dependent/independent anti-tumor activities alone/in combination with gemcitabine.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 1 | 9.22 | 9.22 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| EFO_ID | EFO_ID | - | — | MONDO:0004992 |
| EFO_TERM | EFO_TERM | - | — | cancer |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3301610 | ABEMACICLIB | 4.0 | 1 | 9.22 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3301610 | ABEMACICLIB | Ki | = | 0.6 | nM | 9.22 |