Assay Detail
Binding
CHEMBL5708947
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Antagonist activity at full length rat TRPA1 transfected in HEK293F cells assessed as blockade of MO-induced TRPA1 channel activation by measuring Ca2+ influx in presence of 30 uM MO by FLIPR calcium assay
4
Total Activities
4
Compounds Tested
1
Activity Types
4
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Rattus norvegicus |
| Cell Type | HEK293F |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Transient receptor potential cation channel subfamily A member 1 (CHEMBL5160) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
Species comparison and pharmacological characterization of human, monkey, rat, and mouse TRPA1 channels.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 5.33 | 6.48 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| EFO_ID | EFO_ID | - | — | HP:0012531 |
| EFO_ID | EFO_ID | - | — | HP:0002099 |
| EFO_TERM | EFO_TERM | - | — | pain |
| EFO_TERM | EFO_TERM | - | — | asthma |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3697701 | A-079 | — | 1 | 6.48 |
| CHEMBL1086310 | — | — | 1 | 5.12 |
| CHEMBL593902 | AP18 5 | — | 1 | 4.40 |
| CHEMBL256087 | MENTHOL | 4.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3697701 | A-079 | IC50 | = | 330.0 | nM | 6.48 |
| CHEMBL1086310 | — | IC50 | = | 7600.0 | nM | 5.12 |
| CHEMBL593902 | AP18 5 | IC50 | = | 39900.0 | nM | 4.40 |
| CHEMBL256087 | MENTHOL | IC50 | = | 511000.0 | nM | - |