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Assay Detail

CHEMBL5709948

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PI3Kgamma (unknown origin) using PtdIns(4,5)P2 as substrate preincubated for 20 mins followed by substrate addition and measured after 80 mins by Kinase-Glo assay
1
Total Activities
1
Compounds Tested
1
Activity Types
2
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Characterization of VPS34-IN1, a selective inhibitor of Vps34, reveals that the phosphatidylinositol 3-phosphate-binding SGK3 protein kinase is a downstream target of class III phosphoinositide 3-kinase.
Bago R, Malik N, Munson MJ, Prescott AR, Davies P, Sommer E, Shpiro N, Ward R, Cross D, Ganley IG, Alessi DR.
Biochem J (2014) 463 : 413 -427
PubMed 25177796 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 5.57 5.57

Assay Parameters

Parameter Type Value Units Text
EFO_ID EFO_ID - HP:0000819
EFO_TERM EFO_TERM - diabetes mellitus

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3913746 1 5.57

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3913746 IC50 = 2685.0 nM 5.57