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Assay Detail

CHEMBL5711891

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of JAK1 in human TF-1 cells assessed as reduction in OSM-induced STAT3 phosphorylation by flow cytometry analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
2
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type TF-1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK1 (CHEMBL2835)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

In vitro and in vivo characterization of the JAK1 selectivity of upadacitinib (ABT-494).
Parmentier JM, Voss J, Graff C, Schwartz A, Argiriadi M, Friedman M, Camp HS, Padley RJ, George JS, Hyland D, Rosebraugh M, Wishart N, Olson L, Long AJ.
BMC Rheumatol (2018) 2 : 23 -23
PubMed 30886973 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 8.80 8.80

Assay Parameters

Parameter Type Value Units Text
EFO_ID EFO_ID - HP:0001370
EFO_TERM EFO_TERM - rheumatoid arthritis

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3622821 UPADACITINIB 4.0 1 8.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3622821 UPADACITINIB IC50 = 1.6 nM 8.80