Assay Detail
Binding
CHEMBL5715964
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of His6-tagged recombinant human CDK2/cyclin E expressed in baculovirus infected Sf21 cells using histone H1 as substrate in the presence of gamma-32P-ATP by scintillation counter analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
2
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf21 |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Target
Cyclin-dependent kinase 2/cyclin E1 (CHEMBL1907605) ↗| Type | PROTEIN COMPLEX |
| Organism | Homo sapiens |
Publication
In vitro and in vivo antitumor properties of the cyclin dependent kinase inhibitor CYC202 (R-roscovitine).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 6.81 | 7.00 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| EFO_ID | EFO_ID | - | — | MONDO:0004992 |
| EFO_TERM | EFO_TERM | - | — | cancer |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL14762 | SELICICLIB | 2.0 | 1 | 7.00 |
| CHEMBL133342 | (S)-ROSCOVITINE | — | 1 | 6.62 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL14762 | SELICICLIB | IC50 | = | 100.0 | nM | 7.00 |
| CHEMBL133342 | (S)-ROSCOVITINE | IC50 | = | 240.0 | nM | 6.62 |